“Living drug factories” help treat type 1 diabetes
Thursday, 2 April 2020
Researchers in the US have developed a way to protect transplanted drug-producing cells from immune system rejection. Creating “living drug factories” which could help treat diabetes and other conditions and diseases.
One promising way to treat diabetes is with transplanted islet cells that produce insulin when blood sugar levels get too low. However, patients who receive these transplants must take drugs to prevent their immune systems from rejecting the transplanted cells. So the treatment isn’t often used.
About the research
To help make this type of therapy more feasible, MIT researchers have now devised a way to encapsulate therapeutic cells in a flexible protective device that prevents immune rejection while still allowing oxygen and other critical nutrients to reach the cells. Such cells could pump out insulin or other proteins whenever they are needed.
“The vision is to have a “living drug factory” that you can implant in patients, which could secrete drugs as-needed. We hope that technology like this could be used to treat many conditions, including diabetes,” says Daniel Anderson, Associate Professor Chemical Engineering, MIT and the senior author of the work.
In a study of mice, the researchers showed that genetically engineered human cells remained viable for at least five months, and they believe they could last longer to achieve long-term treatment of chronic conditions like diabetes or haemophilia.
Patients with type 1 diabetes usually have to inject themselves with insulin several times a day to keep their blood sugar levels within a healthy range. Since 1999, a small number of diabetes patients have received transplanted islet cells, which can take over for their non-functioning pancreas. While the treatment is often effective, the immunosuppressant drugs that these patients have to take make them vulnerable to infection and can have other serious side effects.
For several years, Anderson’s lab has been working on ways to protect transplanted cells from the host’s immune system, so that immunosuppressant drugs would not be necessary.
“We want to be able to implant cells into patients that can secrete therapeutic factors like insulin, but prevent them from being rejected by the body. If you build a device that can protect those cells and not require immune suppression, you could really help a lot of people.”
To protect the transplanted cells from the immune system, the researchers housed them inside a device built out of a silicon-based elastomer (polydimethylsiloxane) and a special porous membrane.
They then coated the outer surface of the device with a small-molecule drug called THPT. In a previous study, the researchers had discovered that this molecule can help prevent fibrosis, a build-up of scar tissue that results when the immune system attacks foreign objects.
The device contains a porous membrane that allows the transplanted cells obtain nutrients and oxygen from the bloodstream. These pores must be large enough to allow nutrients and insulin to pass through, but small enough so that immune cells such as T cells can’t get in and attack the transplanted cells.
Drugs on demand
In a study of diabetic mice, the researchers showed that transplanted islets inside microdevices maintained normal blood glucose levels in the mice for more than 10 weeks.
The researchers also tested this approach with human embryonic kidney cells that were engineered to produce erythropoietin (EPO), a hormone that promotes red blood cell production and is used to treat anemia. These therapeutic human cells survived in mice for at least the 19-week duration of the experiment.
“The cells in the device act as a factory and continuously produce high levels of EPO. This led to an increase in the red blood cell count in the animals for as long as we did the experiment,” Anderson says.
The researchers also showed that they could program the transplanted cells to produce a protein only in response to treatment with a small molecule drug. Specifically, the transplanted engineered cells produced EPO when mice were given the drug doxycycline. This strategy could allow for on-demand production of a protein or hormone only when it is needed.
This type of “living drug factory” could be useful for treating any kind of chronic illness or condition that requires frequent doses of a protein or hormone, the researchers say. They are currently focusing on diabetes and are working on ways to extend the lifetime of transplant.